![]() This is used to set the scale of the image and allows determination of the distance between localisations. Specify the size of each pixel in the image. The information that is required is detailed below: This information is used is many of the analysis plugins. The SMLM plugins require information about the system used to capture the images. If the calibration cannot be loaded a wizard is shown to create a calibration (see section 4.1.1.1).Ī dialog is shown to select output options (see section 4.1.2).Įach frame of the image will be analysed and the localisations recorded in a table and/or drawn on a high resolution image reconstruction. Loads the current calibration (see section 4.1.1). The plugin must be run when a single-molecule image is the currently active window in ImageJ. This simplifies the fitting process to a single-click operation. The fitting is performed using the fitting defaults, i.e. The Simple Fit plugin provides a single plugin to fit localisations on an image and produce a table and image reconstruction of the results. The plugins are described in the following sections using the order presented on the Plugins > GDSC SMLM > Fitting menu. Please refer to section Localisation Fitting Method for more details. It is vital that the method used by the software is understood when adjusting the parameters from the defaults. The following plugins use the SMLM fitting engine to find and fit spots on an image. ![]()
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